Isolation and characterization of lipid-protein particles containing platelet factor 3 released from human platelets
نویسندگان
چکیده
منابع مشابه
Characterization of soluble CD40 ligand released from human activated platelets.
We report here that soluble CD40 ligand (sCD40L) is released from human platelets when activated with collagen or thrombin. The sCD40L was detectable in the culture supernatants of platelets within 30 min after stimulation in vitro, and reached maximal levels in 3 h. The release was blocked by the metalloproteinase inhibitor, KB8301, indicating that the soluble CD40L is made by cleaving the mem...
متن کاملIsolation and characterization of plasma membranes from human blood platelets.
Human platelets were subjected to hypoto?ic lysis after being loaded intracellularly with glycerol (4.3 M) by centrifugation in a glycerol gradient under controlled conditions. A homogeneous membrane fraction, free of intracellular organelles, was isolated after a single density step centrifugation, while continuous sucrose density gradient centrifugation gave two subfractions with densities of...
متن کاملFunctional characterization of human platelet-released factor V and its activation by factor Xa and thrombin.
The functional characterization of human platelet-released factor V and its activation by factor Xa and thrombin was studied by functional assessment of cofactor activity and Western blotting analyses of platelet releasates, obtained by stimulating washed suspensions of platelets with various agonists, including collagen, collagen with ADP, and the calcium ionophore A23187. Platelet factor V wa...
متن کاملIsolation and characterization of actin and actin-binding protein from human platelets
Human blood platelets, which are highly motile cells essential for the maintenance of hemostasis, contain large quantities of actin and other contractile proteins. We have previously introduced a method (Lucas, R. C., T. C. Detwiler, and A. Stracher, J. Cell Biol., 1976, 70(2, Pt. 2):259 a) for the quantitative recovery of the platelets' cytoskeleton using a solution containing 1% Triton X-100 ...
متن کاملPurification and characterization of macromolecular phagocytosis activators released from platelets.
Substantial amounts of macromolecular activators of phagocytosis from platelets (MAPPs) were released in response to exposure of platelets to the specific agonists thrombin and collagen and to calcium ionophore A23187. Centrifugation of the platelets in culture medium also resulted in a release of MAPPs, but not when the platelets were frozen and thawed prior to centrifugation. In an experiment...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1982
ISSN: 0264-6021
DOI: 10.1042/bj2030303